Isolation:Article Title: Selective subepicardial localization of monocyte subsets in response to progressive coronary artery constriction.
Article Snippet: .. After isolation of peripheral blood mononuclear cells (PBMCs) by Ficoll density gradient centrifugation, monocytes were probed with a mouse antihuman CD14-FITC monoclonal antibody (AbD Serotec; Clone TÜK4) and a biotinylated anti-human L-selectin (CD62L) antibody (R&D Systems), both at 1:20 dilution for 15 min at 4°C. .. PBMCs were washed with cold EDTA buffer at 350 g for 5 min (4°C), followed by incubation with streptavidin-phycoerythrin (R&D Systems) at 1:20 dilution for 15 min at 4°C.
Gradient Centrifugation:Article Title: Selective subepicardial localization of monocyte subsets in response to progressive coronary artery constriction.
Article Snippet: .. After isolation of peripheral blood mononuclear cells (PBMCs) by Ficoll density gradient centrifugation, monocytes were probed with a mouse antihuman CD14-FITC monoclonal antibody (AbD Serotec; Clone TÜK4) and a biotinylated anti-human L-selectin (CD62L) antibody (R&D Systems), both at 1:20 dilution for 15 min at 4°C. .. PBMCs were washed with cold EDTA buffer at 350 g for 5 min (4°C), followed by incubation with streptavidin-phycoerythrin (R&D Systems) at 1:20 dilution for 15 min at 4°C.
SDS Page:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Molecular Weight:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Membrane:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Article Title: Syk-mediated Tyrosine Phosphorylation Is Required for the Association of Hematopoietic Lineage Cell-specific Protein 1 with Lipid Rafts and B Cell Antigen Receptor Signalosome Complex
Article Snippet: .. The immunoprecipitates and aliquots of cell lysates were resolved by 10% (v/v) SDS-PAGE, transferred to Millipore Immobilon polyvinylidene difluoride membrane, and immunoblotted using appropriate primary antibodies followed by secondary peroxidase-conjugated antibodies (Bio-Rad). .. Blots were visualized with enhanced chemiluminescence (ECL, Amersham Biosciences) and autoradiography.
Incubation:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Functional Assay:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Muscles:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Gene Expression:Article Title: The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Article Snippet: Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating at 95 °C for 5 min. .. Samples were then run on 4-20% gradient SDS-PAGE gels (Mini-PROTEAN TGX Pre-cast gels, Bio-Rad, 4561096) alongside a molecular weight ladder (Precision Plus protein standard, Bio-Rad, #1610374) and transferred to PVDF membranes (Immobilon-P PVDF membrane, Millipore, #IPVH00010), which were blocked with either 5% milk powder or 5% BSA (according to manufacturer’s instructions) for 1 h. Subsequently, the membranes were incubated overnight at 4 °C with the following primary antibodies (all used at 1:1000): mouse anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) (Cell Signaling, #9106), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling, #4511), rabbit anti-phospho-Smad2 (Ser465/467) (Cell Signaling, #8828), rabbit anti-phospho-Akt (Ser473; D9E) (Cell Signaling, #4060 S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; 81E11) (Cell Signaling, #4668 S), rabbit anti-α-Tubulin (11H10) (Cell Signaling, #2125), rabbit anti-SQSTM1/p62 (Cell Signaling, #5114), and mouse anti-Flag (M2) (Sigma, Fig. 7 rFibcd1 reduces transcriptional and functional changes induced by cachectic melanomas. a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n= 9) or treated with rFibcd1 (n= 10), and mice that carry a cachectic melanoma and that are either mock-treated (n= 7) or treated with rFibcd1 (n= 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. ..
Liposomes:Article Title: Scalable Production of Extracellular Vesicles and Its Therapeutic Values: A Review
Article Snippet: , Phosphorothioate (PS) B-class CpG oligonucleotides (ODN 2006PS; 2 μM), S. salar DNA (15 μg/mL), or E. coli DNA (15 μg/mL) , Salmon head kidney leukocytes (HKLs), Atlantic salmon kidney cells (ASK cells), chinook salmon embryo cells (CHSE-214 cells), or HEK293T cells , 1 h (2006PS, S. salar or E. coli DNA) for HEK293T cells , Ultracentrifugation , Densitometry of Western blot (Alix): 10.1-, 16.7-, and 9.1-fold increases in Alix+ EV protein content for ODN 2006PS-treated HKLs, ASK cells, and CHSE-214 cells, respectively; for HEK293T cells, 3.3-, 2.1-, and 9-fold increases in Alix+ EV protein content for 2006PS, S. salar DNA, and E. coli DNA groups, respectively , Not reported , [ ] . .. Cationic bare liposomes (CL: HSPC-based or DOPE-based; 0.5 to 2 mM) or neutral bare liposomes (NL; 0.5 to 2 mM) , Murine colorectal cancer cell line (C26), murine melanoma cell line (B16BL6), human gastric cancer cell line (MKN45), and human colorectal cancer cell line (DLD-1) , 48 h , Ultracentrifugation or precipitation (ExoQuick-TC TM ) , Bio-Rad DC ® protein assay: 2.5-, 2.3-, 1.7-, and 1.8-fold increases in EV protein concentration with 2 mM of NL for C26, B16B16, MKN45, and DLD-1 cell lines, respectively; 3.4-, 3.4-, 3.7-, and 2.9-fold increases in EV protein concentration with 2 mM of HSPC-based CL for C26, B16B16, MKN45, and DLD-1 cell lines, respectively. DOPE-based CLs further increased EV protein concentration (up to 3.17-fold) , Liposome-stimulated EVs showed higher cellular uptake , [ ] . .. Physiological modification , Hypoxic (1% O 2 , 0.1% O 2 , or 1 mM DMOG) , Breast cancer cell lines MCF7, SKBR3, and MDAMB 231 , 24 h (1% O2) or 48 h (0.1% O2 and DMOG) , Precipitation (ExoQuick TM ) , NTA: Up to 1.41-fold, 1.94-fold, and 1.45-fold increases in small EV particle concentration at 1% O 2 , 0.1% O 2 , and 1 mM DMOG, respectively , Highlighted the importance of the study of EV-mediated pathological hypoxic signaling in tumor progression , [ ] .
DC Protein Assay:Article Title: Scalable Production of Extracellular Vesicles and Its Therapeutic Values: A Review
Article Snippet: , Phosphorothioate (PS) B-class CpG oligonucleotides (ODN 2006PS; 2 μM), S. salar DNA (15 μg/mL), or E. coli DNA (15 μg/mL) , Salmon head kidney leukocytes (HKLs), Atlantic salmon kidney cells (ASK cells), chinook salmon embryo cells (CHSE-214 cells), or HEK293T cells , 1 h (2006PS, S. salar or E. coli DNA) for HEK293T cells , Ultracentrifugation , Densitometry of Western blot (Alix): 10.1-, 16.7-, and 9.1-fold increases in Alix+ EV protein content for ODN 2006PS-treated HKLs, ASK cells, and CHSE-214 cells, respectively; for HEK293T cells, 3.3-, 2.1-, and 9-fold increases in Alix+ EV protein content for 2006PS, S. salar DNA, and E. coli DNA groups, respectively , Not reported , [ ] . .. Cationic bare liposomes (CL: HSPC-based or DOPE-based; 0.5 to 2 mM) or neutral bare liposomes (NL; 0.5 to 2 mM) , Murine colorectal cancer cell line (C26), murine melanoma cell line (B16BL6), human gastric cancer cell line (MKN45), and human colorectal cancer cell line (DLD-1) , 48 h , Ultracentrifugation or precipitation (ExoQuick-TC TM ) , Bio-Rad DC ® protein assay: 2.5-, 2.3-, 1.7-, and 1.8-fold increases in EV protein concentration with 2 mM of NL for C26, B16B16, MKN45, and DLD-1 cell lines, respectively; 3.4-, 3.4-, 3.7-, and 2.9-fold increases in EV protein concentration with 2 mM of HSPC-based CL for C26, B16B16, MKN45, and DLD-1 cell lines, respectively. DOPE-based CLs further increased EV protein concentration (up to 3.17-fold) , Liposome-stimulated EVs showed higher cellular uptake , [ ] . .. Physiological modification , Hypoxic (1% O 2 , 0.1% O 2 , or 1 mM DMOG) , Breast cancer cell lines MCF7, SKBR3, and MDAMB 231 , 24 h (1% O2) or 48 h (0.1% O2 and DMOG) , Precipitation (ExoQuick TM ) , NTA: Up to 1.41-fold, 1.94-fold, and 1.45-fold increases in small EV particle concentration at 1% O 2 , 0.1% O 2 , and 1 mM DMOG, respectively , Highlighted the importance of the study of EV-mediated pathological hypoxic signaling in tumor progression , [ ] .
Protein Concentration:Article Title: Scalable Production of Extracellular Vesicles and Its Therapeutic Values: A Review
Article Snippet: , Phosphorothioate (PS) B-class CpG oligonucleotides (ODN 2006PS; 2 μM), S. salar DNA (15 μg/mL), or E. coli DNA (15 μg/mL) , Salmon head kidney leukocytes (HKLs), Atlantic salmon kidney cells (ASK cells), chinook salmon embryo cells (CHSE-214 cells), or HEK293T cells , 1 h (2006PS, S. salar or E. coli DNA) for HEK293T cells , Ultracentrifugation , Densitometry of Western blot (Alix): 10.1-, 16.7-, and 9.1-fold increases in Alix+ EV protein content for ODN 2006PS-treated HKLs, ASK cells, and CHSE-214 cells, respectively; for HEK293T cells, 3.3-, 2.1-, and 9-fold increases in Alix+ EV protein content for 2006PS, S. salar DNA, and E. coli DNA groups, respectively , Not reported , [ ] . .. Cationic bare liposomes (CL: HSPC-based or DOPE-based; 0.5 to 2 mM) or neutral bare liposomes (NL; 0.5 to 2 mM) , Murine colorectal cancer cell line (C26), murine melanoma cell line (B16BL6), human gastric cancer cell line (MKN45), and human colorectal cancer cell line (DLD-1) , 48 h , Ultracentrifugation or precipitation (ExoQuick-TC TM ) , Bio-Rad DC ® protein assay: 2.5-, 2.3-, 1.7-, and 1.8-fold increases in EV protein concentration with 2 mM of NL for C26, B16B16, MKN45, and DLD-1 cell lines, respectively; 3.4-, 3.4-, 3.7-, and 2.9-fold increases in EV protein concentration with 2 mM of HSPC-based CL for C26, B16B16, MKN45, and DLD-1 cell lines, respectively. DOPE-based CLs further increased EV protein concentration (up to 3.17-fold) , Liposome-stimulated EVs showed higher cellular uptake , [ ] . .. Physiological modification , Hypoxic (1% O 2 , 0.1% O 2 , or 1 mM DMOG) , Breast cancer cell lines MCF7, SKBR3, and MDAMB 231 , 24 h (1% O2) or 48 h (0.1% O2 and DMOG) , Precipitation (ExoQuick TM ) , NTA: Up to 1.41-fold, 1.94-fold, and 1.45-fold increases in small EV particle concentration at 1% O 2 , 0.1% O 2 , and 1 mM DMOG, respectively , Highlighted the importance of the study of EV-mediated pathological hypoxic signaling in tumor progression , [ ] .
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